Theater of parallel Universe

Brook Sun Dec 23, 2012 11:20 am

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°251

Re: Theater of parallel Universe

Brook Sun Dec 23, 2012 11:20 am

So much to cover and where to begin...so I guess the beginning. Whoa! There's that word again with the three n's and the primer.

The beginning of this I guess you could call it started with a four day download. Now there is much more detail to this that I won't bore you with, but I have to get this part out in the open for you to see how it connects the dots to what I found last night.

I believe I mentioned in the beginning that the word "Ptah" set it all off. Prior to that I also mentioned I had no awareness of anything remotely Egyptian. For four days information was coming into my awareness in the form of block pixels...when that started so did the lines on my hands. This is an important part. I have around 280 photos last count of various lines on my hands some darker than others some pin point. some parallel lines as in two going the same direction. And the first impression I got and was validated by another who had some experience saw a "Druid" connection to the lines as symbols...or partial symbols.

Now as time went on I figured out and validated lots of parallel to what I was seeing and the energy signatures given were accurate but something was missing in part that I questioned..but did not feel was all that important. I figured the lines on my hands were a stressor mark of some sort. Residual memory. And in fact that is the case for the better part. But I never connected it in a validating sort of way.

I knew about the past in Scotland and the Egyptian connection. The stone of Destiny being brought here by Queen Scota and the flag she hoisted with the staff of Moses painted on it. But did not have a solid foundation to connect it other than I knew I was following the back engineered technology brought from Egypt to Scotland. Until last night I never even saw a picture of the Stone of Destiny....I was just made aware of the connection to my being there in Scotland at that time.

I have to say I've run into about seven people that are involved in this scene and I've met them in this current life stream as in the now. For example. I've met the boy I was going to heal and thought had died when I was plunging to my death. In fact he did not die. He lived and was deeply disturbed at my death and the connection I had to his father..not as his mother..but mistress. You see the boy had a horrible self centered mother who was far form being a fit mother...and I was his best friend. He looked up to me and knew of my association to his father. When he heard of my death he went mental and being from a somewhat royal lineage he waged war with another clan he figured was responsible for my death...along with his father. Now as far as I know I don't have any healing abilities now..but my hands were important for a reason. Which you'll see in another post coming up.

Now the "beginning"...the primer. The three wavy lines. just a week or so ago I saw them as a "primer". But I had seen them before. Which I will explain in another post. Just know that the three wavy lines were not "new" to me....just the association as a primer was brought to my awareness this month. I believe it was the ninth of this month as I started back doing my sessions after a break I took.

To recount. The lines on my hands are important here and the Stone of Destiny. The staff on the flag of Queen Scota plays a part here as well to what I discovered last night.

Now what I've not told you here is that the day of the ritual was also the day of my death in Egypt. I was brought to Egypt form Atlantis for a reason. My association to the disc technology. I knew this..but it was not until last night I was made aware of exactly how...other than I was made aware of a genetic disposition and connection to the discs and where they are from. The expedition and it's demise. I played a part in seeing that they were successfully returned where they belonged. But there was something I could not connect until last night.

Ive noted to you that the technology in this room and the ritual was back engineered. Even the chair was a back engineered version. As I've explained that I've used one of the original ones to sit in and create a vortex of rose color and gold specs. That chair was much different than the back engineered one. I did not realized how much until last night. I just assumed it was operationally the same. It was not. This part will interest you in the coming post as it involves your black ship heavily I believe. In fact I believe the white ship you view is the chair. I may be incorrect in this thinking... but when you see what I've discovered you may see a similarity.

I've closely read what you've written and see many parallels in fact. So keep an open mind here and allow me to expalain.

The night of the ritual...I was forced to participate in this ritual. Otherwise they would kill my family which they had in custody. They also promised to kill my lover. So I went along with it to protect them. I knew and had seen the abomination they would try to create genetically and I assumed they needed my assistance in this field..as that was after all my field. I assumed wrong. You see they tried to assimilate what we were doing with the original discs and made the most horrific mistakes..and the pain they were creating in these things they were creating was extreme. Now you ask why I stayed after the discs were gone. Well it was not my plan to stay long...in fact I had an exit plan in the ready. But they found out and held my family hostage and my lover. They needed me. I found out exactly why last night. This has always been a question in my mind but until now I simply rationalized it.

After the ritual which was not creating an abomination but another use entirely....I went to my room. I had a friend watching over me sent by my lover for that very reason and he gave me a dagger to protect myself. He kept close watch over me in the absence of my lover who was in fact in another country. He came to me after the ritual to see how I was and he reported in fact that they had lied and actually killed my lover. At this point he went to get me something to relax me as I was in shock from not only the ritual but the news of his death. When he came back I was standing in the middle of the room and had a stab wound in my abdomen, blood dripping down to the floor and the dagger on the floor next to me. He called for help and it created a real stir. The one in charge of the ritual and in power at the time...came storming in. He was furious. He was Ptah. Not a god..but to be presumed as such based on the "magic" and powers he possessed. He had me dragged out of the room and down several corridors to an area where there was a snake pit. He yelled at me told me I could have had the world...then threw me in the pit. He finished me off.

Okay then...now you can see why the emotional attachments I had to over come to see what was going on. Why I was stuck in that damned room. Now I'm actually fine....so no concerns with the emotional attachments. I must confess I was a bit concerned last night when I started but it was actually quite easy to disconnect with the emotional and inspect the operational surroundings better. This is the short version. I can actually provide lots of validation to this story as well as when I first became aware of it..I actually thought I was losing my mind. But the more I worked on it the more validations I got. Particularly when I finally decided to do a study on this ancient Egyptian past and so called "myth". Why the science of it is actually proof...I've been shown things I had no idea existed genetically, atomically and mythical based facts that are simply to coincidental to ignore.

Last edited by Brook on Sun Dec 23, 2012 12:25 pm; edited 1 time in total

Brook Sun Dec 23, 2012 11:23 am

Brook

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Post n°252

Re: Theater of parallel Universe

Brook Sun Dec 23, 2012 11:23 am

I should start by repeating that when I first came upon this information I was gathering I waited six months before I started to research some of the things I was seeing. For the greater part because I was sure I was losing my mind. It was like this story of people and events of which I had no prior knowledge was surreal. The two people I was relating this to were amazed at the things I was bringing up as they were in fact looking up these things and they made it clear that I not look anything up on the internet. So I did not. Not until after that ritual and started having flashes of scenes in my conscious state did I decide to finally relent and research what I was finding. Then it became even more strange. The more I would find the more it validated. Now at this time I have to say....as I did before....there is a hidden part I will not discuss on the internet. But it is the most telling and validating proof of all. It's the smoking gun. And because of this I usually don't present as much as I have here. But because of the relevance to the facts that I have discovered such as the genetic disposition and it's manipulation that effects everyone I have no problem discussing what I have so far. Particularly the scientific proof that coincides with all of this.

I have to discuss two more factors of what I have already discovered to put it into perspective with what I saw last night. On that note when I first realized this room before I ever witnessed the ritual....my first impression was this room was used for mummification among other things. With that my first thought was...where is this room located? Upon asking that question I discovered it is currently under water. I viewed it. Now the room is empty except for one thing..that is current as in the now time frame. So somewhere in Egypt is a room under water and this black stone block....highly polished sharp edges....and it's accessible in the now with the right equipment to go searching underwater.

My first question then was ...why is the block still there? Until last night I had no idea. The chair was gone...the disc was gone...but the stome block was still there? I know now.

The other thing I want to review is the first part of the ritual. You see as an unwilling participant they wanted to be assured that I cooperate. So the first part of the ritual which was quite extraordinary...involved my prep. Which included being given doses of the Blue Lily and poppy flower together to drug me into submission. Not too much just enough. Then I was prepared by two servant types..a man and a woman. After a ritual bathing in a pool with this same flowers floating in it, I was taken to an area where I was bathed in smoke of amber resin..and anointed with oil from head to toe. Then a man sitting to the left who was watching stood up with this staff and proceeded to preform some kind of ritual upon which I met the owl...I was taken over and was not in the drivers seat anymore.

After viewing the ritual I understood why I needed to be possessed by this owl. But it was much more than that. Remember...the guy had a staff....it was a form of basic electronic mind control. The owl was the perceived "BA" of the Egyptians. But it was administered by the man with the staff and I believe the oil that was used on me some kind of conductor to maintain that electronic mind control to preform my duties as it were in the ritual. ...once that was achieved the real ritual occurred.

Back to the block....upon my first seeing it I noticed it was simply a black stone. But something told me to wave my hand in front of one end..that is when I saw the primer....the three wavy lines. Last night I discovered upon further inspection if I did that in other areas of the stone block...other symbols popped up. Simply by waving my hand in front of various areas of the block. And they coincided with the lines I've been getting on my hands. Like a mirror effect. What finally dawned on me is this.....That was how you activate the block. And I had the codes mirrored in my hands. I was in fact the only one who could activate that block. This is why they needed me to cooperate and held my family as hostage to be assured I would assist. Genetic disposition to the block...which was in fact part of the original set up of the original disc technology. Only the chair and disc were back engineered.

This is the reason that block is still in that room underwater. With me dead it was useless to them. that was a big hit for me last night. I could not figure out why they left the bock behind in that room. But I was aware I had something they needed to assist in the ritual...I was just not certain what that was until last night. Activating the block with the codes in my hands.

Next post the chair and the staff.

Brook Sun Dec 23, 2012 11:26 am

Brook

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Post n°253

Re: Theater of parallel Universe

Brook Sun Dec 23, 2012 11:26 am

My attention turns to the disc now. But there is not much you can gain from that until it is actually operational. At this point I have to mention I did not view the second part of the ritual. I decided to simply inspect the item in the room in more detail. I'll have to go into another session to view it actually operating. I decided I could not inspect the items while they were in operation to the degree I did. Which turned out to be the best method of inspecting them first.

So I turned to the chair. This chair looked more like a throne. Very ornate with jeweled stones place in various places upon it. It did not look like the one I was familiar with. The one I operated the vortex in on several occasions. At some point I'll draw it out. But the arms were not as wide as the one I'm familiar with and the stones on the arm are different. I detected a thin copper wires within the arm leading to the back of the chair. Then my attention was directed to the staff.

Now this caught my attention...I realized in the original chamber where we used this technology there was never a staff. There was a palm size devise that when placed in the hand was passed over the person and another was operating the panel to adjust the frequency for sending coding to any damaged areas for healing. But never a staff.

This staff was a slender round metal rod. Very simple looking with a flower design at the top. My attention turned to the top and I viewed a green glowing sphere in the center of the flower. It was not actually visible....and the green glow was simply my view to gain my attention to it. I don't believe it actually glows green. The only way you can actually view this sphere is by looking at it from the top and seeing the top part of the sphere which is embedded in the flower design. Then my attention was drawn to the bottom of the staff. It flanged to a triangle shape.

That is when I noticed another stone set at the foot of the chair. With two hole in it. and in the holes was a metal lining that would accommodate the triangle bottom of the staff. so I gather you have to put the staff in it to operate. But I've not seen it operate just yet so I'm just assuming. This stone was like a foot rest at the bottom of the chair. This is when my attention was grabbed. This is the "Stone of Destiny" that resides in Scotland now I believe. this is the stone that Queen Scota brought with her. Then I realized her flag had a staff on it. Oh boy...the plot thickens!

So after the session I went to research a few things....I looked up the "Stone of Destiny" to see if I could get a picture.....and here it is...with two holes in it!

The Celtic name of the Scottish Coronation Stone is Liath Fàil, "the speaking stone" because it named the king it would soon enthrone, and might sing or roar with joy when he sat on it!

Within the holes of this stone I saw a metal insert where the staff would rest. Needless to say when I saw this picture I about fell off my chair!

Now there were several staffs in Ancient glyphs depicted...but I want to bring attention to the one that you mostly see Isis sporting. As she was the one I've determined that dealt with the genetic disposition to utilize this method of application regarding "genes". this would be a good likeness of the staff I saw used with the chair. Genes....Genesis ....Genes of Isis.

And who wore a "Throne" on her head?

But this was NOT Isis in this ritual ceremony. It was a man who sat in the chair and called for his staff when it first started.

Okay next the most unusual part that simply blew me away. The back of the chair.

Last edited by Brook on Sun Dec 23, 2012 1:59 pm; edited 1 time in total

Brook Sun Dec 23, 2012 11:28 am

Brook

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Post n°254

Re: Theater of parallel Universe

Brook Sun Dec 23, 2012 11:28 am

The back of the chair. I would like to take this time first to say I am not an engineer. when I first glanced at the field generator I was at a loss to explain what I was viewing. So often times I have to gain energy signatures....as I'm completely clueless as to what I'm looking at. That being said, I was completely lost on the back of the chair.

Now looking at the back of the chair I noticed the stones (jewel like) on the top facing of the back of the chair. Looking at it you would most certainly think you were viewing a very ornate throne. I will have to draw it give you a better view. That beings said in the center of the back was a rectangle area of what appeared to be a leather cushioned pad that ran from the seat of the chair to about eight inches from the top of the jewel lined area. Upon viewing this I decided to pull the cushion off as I determined there was something behind it.

There is where I viewed a complex machinery I was completely clueless as to what it was. What I first thought of was it sort of reminded me of the back of an old refrigerator I had seen a long time ago. On the top of the machinery was a part that looked almost like an accordion...metal stacked in lines. and it was the width of the cushion. in the center it had like a cut out area of about one inch and I have no idea why it was that way.

At the bottom were three visible tubing's that flanged to what I describe as a metal bottle that would appear to hold some kind of gas? Mind you this is simply a frontal view. I was clueless as to what I was looking at I kept asking for an energy signature which I did get and what I got was "hydrogen"...then in a verification I asked again and got "cell". Now at this point I'm lost....I have no idea how it works what it does but it seems very complex to someone like me who is not an engineer nor do I have the expertise to understand how hydrogen plays a part here. I then thought and this is weird....I've seen a similar thing on the top part that I may have seen on a motorcycle...but again ....clueless as I am.....I have no idea which part of a motorcycle it would be..I just associated it with something I kind of remembering seeing in that area of a motorcycle.

All I can gather with an assumption is it somehow powers this chair. The other chair I'm aware of is not even close to this....there is no machine built into the one where I created the vortex. Period.

So at this time I decided to end the session and see if I could figure out what I was seeing. I first looked up that stone of Destiny which rocked me. Then I looked up hydrogen cell.....and it blew my mind! This picture is extremely close to what I viewed....the one inch cut out is not there but if you turn this devise sideways the things that are projecting out of them would certainly fit the the bottle looking things that were placed at the bottom. In other words..the metal stacked is just sideways to this picture.

http://en.wikipedia.org/wiki/Fuel_cell

When I goggled hydrogen cell I saw fuel cell in wikipedia and it's so close to what I viewed. Apparently there is a fuel cell that is run on hydrogen as well which I was unaware of. I believe this picture is not a hydrogen cell....but it's the same principal. the center part with the rows of metal I could not see the very top..but it could have had connecting devises on it. And I did see a copper wire within the arms of the chair briefly.

So there you have what I viewed that set me spinning. It not only brought some interesting realizations as to the origins of the back engineered model...it tied some lose ends that I had previously not understood but questioned. In particular the block left behind in that room that is currently underwater.

I will be going back to view the operation of these items....but to do that I have to view the ritual. Just so you know.

Brook Sun Dec 23, 2012 11:56 am

Brook

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Post n°255

Re: Theater of parallel Universe

Brook Sun Dec 23, 2012 11:56 am

Now...the conversations got into depths for which I cannot bring here.

But at that last sentence...I did not go back to the ritual. Too much emotional attachment at the time to address. It was quite frankly horrible.

But mentioning the room is now under water....

The disc, the chair, the field generator, and the dejeds are gone. Removed. But the Stone block does still remain.

If the other items were there...and the water level was gone...turned on so to speak....you could wave your hands over the stone block in certain areas....and there would be symbols appear.

That is what I did in the ritual. waved my hands over the symbols to activate it for the ritual.

The ritual took place here. It flooded some back then and still today. There was a drainage system set up back then....one part of that system...right off the "room". The Osirian Temple:

Where this symbol also resides:

So what causes these black lines?

A hint is in this video...the black goo...

In this re-upload of the ET Falklands War ET connections, David Griffin of Exopolitics UK sets the stage for the details of a Blue ET-Base on Thule Island south of the Falklands, the Black Goo or Gray Goo, an "intelligent oil" allegedly leading to the deaths of many staff at MARCONI in the UK.

Coming soon...the information I was presented the other day...message in the music videos I present words and video..how does this one open? The song....The beginning....water...flow/action...the three N's....

Brook Thu Dec 27, 2012 9:08 am

Brook

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Post n°256

Re: Theater of parallel Universe

Brook Thu Dec 27, 2012 9:08 am

Good morning

I do hope you had a chance to review the assumptions on the "black goo". I say assumptions because here you have to assume what they are telling you. Quite frankly this is what I am going to talk about in the next few references.

You can only relate what you've been given as information toward a particular part of the puzzle. Given intricate details and blocks of information leading toward a conclusion can be utterly confusing. Particularly to a 3D mind set. I know I have struggled with it in immense proportion. This is why I started off this thread with the 180 turnaround. You can only relate some things is such a way. Once you get past that and work to understand there is another totally different way to look at the intricate details of information in another light it becomes quite different.

Have I confused you yet? Hang with me here as I work to discuss some of the intricate details of the Discs.

But before I do I want to revisit an older video I used to present as a piece of the puzzle of information with it's intricate details which are not fully explained.....yet OH SO CLOSE....

I'd like to say welcome back Mercuriel....as you are a key brother and we've missed you!

From my heart to yours Brother.

Hadriel

Last edited by Brook on Thu Dec 27, 2012 10:48 am; edited 1 time in total

Brook Thu Dec 27, 2012 10:24 am

Brook

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Post n°257

Re: Theater of parallel Universe

Brook Thu Dec 27, 2012 10:24 am

Lot's of information packed into that little video...with great music I might add.

Note: Once you separate yourself from the light that is that vehicle you will make it quite difficult to achieve that "activation". Once you "equate" it to a vehicle...and not "you"...you automatically separate it.

two "separate" tetrahedra..with a "common center".

That common center is you....that light is you...always has been. There is no "higher self"...it's always been you...that common center. You are not separate from it...you are not floating out there somewhere...you ARE the common center. In that signature .... is the signature of this universe.

Once you simplify it and not separate it...it becomes one....Not separate...not "out there" "floating" in your higher self..but one with the universe.

Now I sound like a love and lighter eh? I do? Because I do not subscribe to the "higher self". That was quite frankly one of the blocks of information I received. It separates you..and it does that with one simple tool....TIME.

So what does that have to do with the DISCS? Whistle

Brook Fri Dec 28, 2012 9:18 am

Brook

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Post n°258

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 9:18 am

Sorry for the pause...I had meant to go on yesterday. But stopped. Why? Because I decided to get back on the horse. Drop the fear and ride as it were.

So I did a guided session. I did a target specific....then wrote down a list of questions.

Man did I hit a mother load of information.

Interestingly it's connected to the discs also in a strange way.

You see I had a whole section of time I was afraid to decipher. Yet I knew I needed to get the answers. Well not only did I get them...I know now.

This is for you.

I'm not certain if anybody has noticed but when I start posting here on this thread roughly a month and a half ago?...within a 24 hour period I usually get roughly 150 hits or more. When I came back roughly a month and a half ago...I had roughly around 850 hits total...

Just since yesterday when I started posting I've gotten over 150 hits.

Somebody is reading this with great interest eh? I'm certain the folks of the Mists are not hitting it that often.

In part what I got was golden...as in a ratio. Golden Slumbers.....REPOSE....among other things. And how did I do that? I went back to the beginning.... Wink

Here is a song....just for YOU. I'm gonna sing a lullaby....

Brook Fri Dec 28, 2012 10:16 am

Brook

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Post n°259

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 10:16 am

Some of what I will post now will be most likely very confusing. Meant for the ones reading here. But make no mistake I will post some interesting dot connecting information.

This post is about birthright and baby stealing....

What's done is done and this is for you. Told ya I was good at RV.

Brook Fri Dec 28, 2012 10:27 am

Brook

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Post n°260

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 10:27 am

Interesting group Pantera...not to be confused with Panthera:

Genus of BIG CATS.

Panthera is a genus of the family Felidae (cats), which contains four well-known living species: the tiger, the lion, the jaguar, and the leopard. The genus comprises about half of the Pantherinae subfamily, the big cats. The word panther, while technically referring to all members of the genus, is commonly used to specifically designate the black panther, a melanistic jaguar or leopard, and the Florida panther, a subspecies of cougar (Puma concolor coryi).

Only the four Panthera cat species have the anatomical structure that enables them to roar. The primary reason for this was formerly assumed to be the incomplete ossification of the hyoid bone. However, new studies show that the ability to roar is due to other morphological features, especially of the larynx. The snow leopard, Uncia uncia, which is sometimes included within Panthera, does not roar. Although it has an incomplete ossification of the hyoid bone, it lacks the special morphology of the larynx

http://en.wikipedia.org/wiki/Panthera

Roar lion roar!

Brook Fri Dec 28, 2012 10:56 am

Brook

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Post n°261

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 10:56 am

Man I tell ya...Lions and tigers sleeping together....creating natural hybrids!

http://en.wikipedia.org/wiki/Liger

Better yet check this guy out...

Dionysys riding a panther,a maenid, and a satyr boy moving
Dated to between 370 and 360 BC
Currently in the possession of the Museum de Louvre, Paris

Mortals and Gods sleeping together? Holy Cow!... What is this world coming to?

Dionysus had a strange birth that evokes the difficulty in fitting him into the Olympian pantheon. His mother was a mortal woman, Semele, the daughter of king Cadmus of Thebes, and his father was Zeus, the king of the gods.

http://en.wikipedia.org/wiki/Dionysus

Whoa!!!!!!!!!!!!!

Brook Fri Dec 28, 2012 11:10 am

Brook

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Post n°262

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 11:10 am

If you asked the folks producing Ancient Aliens....they think the GODS are Aliens eh?

Funny that.

Anyhow....

Let's take a Sirius look at Genetic Engineering....and the strange things I recovered in my session last night.

Brook Fri Dec 28, 2012 11:42 am

Brook

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Post n°263

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 11:42 am

First off...it was like pulling teeth to get this information. Why? The one giving me the information was scared out of their wits of my reaction. Afraid I would blow a gasket or something. Even Artemis thought it was a VERY bad idea. But over and over again I reassured them I was calm and would remain so.

I won't share everything....I can't... I won't.

So to the reader...rest assured your secret is safe.

Well...some of it anyway. I will bring here some parts that justified delayed, falsified documentation which I possess. And others are aware of....so don't mess with me eh? I don't mean you any harm...but I will tell the truth as it's been presented to me. Truth that I feel others need to hear for some reason I really can't explain.

So...here goes:

I received a series of information blocks. In those blocks were various series of words. Words that were instructed to be written down and researched by me.

Now I posted this video for a reason....Once there was a way to get back home. Sleep pretty darling do not cry....I will sing a lullaby.

Listen again once more to the series of songs in this video while I bring you the various series of words I got last night. Boy...you're gonna carry that weight a LONG TIME eh? Are you going to be in my dream tonight?

Brook Fri Dec 28, 2012 12:10 pm

Brook

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Post n°264

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 12:10 pm

Now keep in mind...I've been called to look into genetic disposition....but have not had any training in the field. So this first series of words was a bit foreign to me. Or was it?

Genetic Adapter:

An adapter in genetic engineering is a short, chemically synthesized, double stranded DNA molecule which is used to link the ends of two other DNA molecules. It may be used to add sticky ends to cDNA allowing it to be ligated into the plasmid much more efficiently.

Adapters are used to link the ends of two DNA molecule that have different sequence at their ends. A conversion adapter is used to join a DNA insert cut with one Restriction enzyme say EcoRl with a vector opened with another enzyme, Bam Hl.This adapter can be used to convert the cohesive end produced by Bam Hl to one produced by Eco Rl or vice versa.

http://en.wikipedia.org/wiki/Adapter_%28genetics%29

So what the heck is EcoRl ????

EcoRI (pronounced, "eco R one") is an endonuclease enzyme isolated from strains of E. coli, and is part of the restriction modification system.

In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.

USES:

Restriction enzymes such as EcoRI are used in a wide variety of molecular genetics techniques including cloning, DNA screening and deleting sections of DNA in vitro. Restriction enzymes like EcoRI that generate sticky ends of DNA are often used to cut DNA prior to ligation, as the sticky ends make the ligation reaction more efficient. EcoRI can exhibit non site-specific cutting, known as star activity, depending on the conditions present in the reaction. Conditions that can induce star activity when using EcoRI include low salt concentration, high glycerol concentration, excessive amounts of enzyme present in the reaction, high pH and contamination with certain organic solvents

Lac Repressor

Protein (green) looping the

DNA at the lactose genes on

E.coli host chromosome

Theater of parallel Universe - Page 11 Lac-DNAloopsmall

Theater of parallel Universe - Page 11 Lac-DNAloopsmall

Theater of parallel Universe - Page 11 599876_396464317080008_1182841555_n

Theater of parallel Universe - Page 11 Isisknot

Theater of parallel Universe - Page 11 Pse

Theater of parallel Universe - Page 11 Blue-light-ankh

Brook Fri Dec 28, 2012 12:24 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°265

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 12:24 pm

Whoa!!!!!...look at that....50 hits already...Makes we wanna shout

Brook Fri Dec 28, 2012 12:28 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°266

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 12:28 pm

Who's my daddy?

funny thing....he was shot in the head. Buried in a cemetery....dug up a few years later...then cremated and put back in the same spot in the vary same cemetery.

I guess I am a little angry after all.

Brook Fri Dec 28, 2012 12:43 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°267

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 12:43 pm

Oh there's more...I'm going to take a break and be back later after that last post. Let's see how many hits it gets now.

Brook Fri Dec 28, 2012 1:37 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°268

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 1:37 pm

Oops..25 more hits....but that should slow down now...

MEMORANDUM: Select data should be copy and paste for review. Initiate has detected signal transmission of data. Transfer and share data to appropriate authority. End transmission.

Aquaries1111 Fri Dec 28, 2012 2:15 pm

Aquaries1111

Aquaries1111: Posts : 1394
Join date : 2012-06-02
Age : 55
Location : In the Suns

Post n°269

Re: Theater of parallel Universe

Aquaries1111 Fri Dec 28, 2012 2:15 pm

Brook Fri Dec 28, 2012 2:31 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°270

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 2:31 pm

Next series of words:

Reactive Hemoglobin:

High-level production of recombinant sulfide-reactive hemoglobin I from Lucina pectinata in Escherichia coli. High yields of fully functional holoprotein synthesis in the BLi5 E. coli strain.

Abstract

Hemoglobin I (HbI) from Lucina pectinata is a monomeric protein composed of 143 amino acids with high sulfide affinity. Its unique heme pocket contains three residues not commonly found in vertebrate globins: Phe 29 (B10), Gln 64 (E7), and Phe 68 (E11), which are thought to be important for high affinity for hydrogen sulfide. Recombinant HbI (rHbI) and several site-directed mutants were cloned and expressed in Escherichia coli yielding high amounts of protein. The highest rHbI protein yield was obtained when the HbI cDNA was cloned into the pET28 (a+) expression vector, transformed into BLi5 cells, the induction performed with 1 mM IPTG at 30 degrees C and TB medium was supplemented with 30 microg/mL hemin chloride and 1% glucose. The highest yield obtained of HbI was 32 mg/L of culture using Fernbach flasks. UV/Visible spectral analysis showed that rHbI binds heme and ESI-MS shows that its molecular weight corresponds to the expected size. Kinetic studies with H2S confirmed that rHbI and HbI have identical binding properties.

http://www.ncbi.nlm.nih.gov/pubmed/15555934

So what is rHbI protein?

Preparation, characterization and photoinduced long-range electron transfer in ruthenium-modified derivatives of recombinant Hemoglobin I (rHbI) from Lucina pectinata

Eladio J. Rivera, eladioj5@yahoo.com1, Luis Rosas1, Ruth Gretchen León, ruthchen@yahoo.com1, Carmen L. Cadilla, ccadilla@rcm.upr.edu2, and Jorge L. Colón, jorgecr@caribe.net1. (1) Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, P.O. Box 23346, San Juan, PR 00931, (2) Biochemistry Department, University of Puerto Rico School of Medicine, San Juan, San Juan, PR 00936

The objective of this work is to prepare an appropriate donor-acceptor system to investigate intramolecular long-range electron transfer (ET) in Hemoglobin I (HbI) of the tropical clam Lucina pectinata. HbI is a monomeric protein composed of 143 amino acids with high sulfide affinity. Its unique heme pocket contains three residues not commonly found in vertebrate globins: Phe29(B10), Gln64(E7), and Phe68(E11), which are thought to be important in ligand binding dynamics. The approach that has been taken in this work is to covalently attach a redox-active ruthenium complex to the surface histidine of HbI. Since this surface histidine is not solvent accessible in met-HbI, recombinant HbI (rHbI) and several site-directed mutants were modified with Ru(bpy)2CO3. Site-directed mutagenesis and the expression of rHbI is an excellent tool for the study of this protein-structure function relationship of this unusual hemoglobin. Recombinant HbI (rHbI) and several site-directed mutants were cloned and expressed in Escherichia coli yielding high amounts of protein. This study presents the purification of modified (His)6-tagged rHbI and several site-directed mutants, in which surface histidine residues (HisX) for ruthenium labeling have been introduced at positions Ser48, and Ala98.Modification experiments were monitored using UV-vis spectrophotometry by comparing the absorbance ratio at 292 nm and 486 nm. The modified met-rHbI and mutants were purified using ion-exchange chromatography on a Fast Protein Liquid Chromatography (FPLC) setup. Intramolecular long-range ET from the Ru(bpy)2(im)(HisX)-excited state to the heme ferric iron in rHbI was monitored by luminescence lifetime measurements. The results of these investigations will be presented.

http://oasys2.confex.com/acs/229nm/techprogram/P831988.HTM

Mutants you say?

....which are thought to be important for high affinity for hydrogen sulfide.

Hydrogen bombshell: Rewriting life's history

Oxygen is supposed to have driven the evolution of complex life – but the discovery of animals that thrive without it tells a different story

GO WEST, young man! More specifically, go about 200 kilometres west of Crete, then straight down to the bottom of the Mediterranean Sea 3.5 kilometres below. There you will find a lake with some extraordinary inhabitants.

Around 6 million years ago when the Mediterranean nearly dried up, vast amounts of salt were deposited on the sea floor. Some of these deposits were exposed about 30,000 years ago. As this salt dissolves, extra-salty, dense water is sinking to the depths, forming a brine lake up to 60 metres deep. Even more surprising than the existence of this lake beneath the sea, however, is what lives in it.

The water in the brine lake does not mix with the water above and so ran out of oxygen ...

If you are a professional diver you should visit Cenote Angelita, Mexico.
There's some kind of cave. When you go deeper than 30 meters the water is fresh, deeper than 60 - it's salty.
And then you see a river underneath, with trees and leaves.
But of course, it's not a real river.
It's just a layer of hydrogen sulphide.
But it looks amazing.

http://www.newscientist.com/article/mg20727721.400-hydrogen-bombshell-rewriting-lifes-history.html

http://acidcow.com/pics/5546-underwater-river-in-mexico-4-pics-video.html

Brook Fri Dec 28, 2012 3:07 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°271

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 3:07 pm

What is H2S protein? One study...

Kinetic studies with H2S confirmed that rHbI and HbI have identical binding properties.

Something rotten never smelled so sweet

Unexpected signaling role for foul-smelling hydrogen sulfide in cell response to protein misfolding

This is what members of a team of scientists at Cold Spring Harbor Laboratory (CSHL) are telling one another as they discuss a new finding they did not expect to make. They have discovered that hydrogen sulfide (H2S) – the flammable, highly toxic gas that we usually associate with the smell of rotten eggs in landfills and sewers – plays an important role in the regulation of a signaling pathway implicated in biological malfunctions linked to Alzheimer's and Parkinson's diseases, among others.

"H2S comes under the category of things that people think of as toxic and nasty, but which can actually be harnessed to serve a useful purpose," says CSHL Professor Nicholas K. Tonks, FRS, who led the research team. In fact, H2S, which is produced naturally in small quantities in various tissues, is a gasotransmitter, one of a family of gaseous signaling molecules that includes nitrous oxide (NO) and carbon monoxide (CO). Unlike growth factors, cytokines and hormones that act through receptors in the cell membrane, these gasotransmitters are able to permeate membranes and enter freely the interior of living cells.

Tonks and colleagues were intrigued by reports in the scientific literature suggesting that H2S was produced as part of the cell's response to what is called ER stress. The ER is the cellular organ called the endoplasmic reticulum. It is an extensive network of membranes spread throughout the cytosol, which is involved in protein synthesis and processing.

When the cell is placed under stress, specifically when newly formed proteins are being manufactured in the ER so rapidly that they do not fold properly, rendering them non-functional, the cell must make a decision either to slow down protein production to match its physiological requirements in the hope that proteins will begin to fold properly or, if that is not sufficient, to commit a form of suicide called apoptosis.

The surprise in the research performed by Tonks' team – which is published online today in Science Signaling – is that H2S plays a critical role in the exquisitely tuned signaling pathway through which cells make this fateful determination.

Navasona Krishnan, a postdoctoral fellow, performed an experiment to determine whether H2S could covalently modify an enzyme called PTP1B. Discovered by Tonks in 1988, PTP1B is a protein tyrosine phosphatase, or PTP – an enzyme that specifically removes phosphate groups from amino acid residues called tyrosines. This function is critical in regulating cellular signaling in normal and disease conditions.

H2S did indeed modify PTP1B, specifically on a cysteine amino acid residue in the enzyme's active site, which inactivated the enzyme. A number of subsequent experiments performed in collaboration with CSHL Professor Darryl Pappin, who directs the proteomics Shared Resource at the Laboratory, identified this modification and revealed that it occurred in vitro and in vivo.

Because PTP1B is itself a signaling pathway regulator, this inactivation was immediately understood to be important and potentially useful. Further experimentation revealed that the H2S-induced modification to PTP1B prevented this phosphatase from inactivating an enzyme called PERK, which is a sensor of the presence of unfolded proteins and a critical regulator of the cell's response to ER stress.

The completed puzzle is as follows: small amounts of hydrogen sulfide are produced when the cell senses ER stress; PTP1B undergoes a unique covalent modification at its active site in response to the H2S that is produced, which in turn prevents the phosphatase from dephosphorylating PERK thereby allowing the latter protein to play its specific regulatory role in response to the stress. Importantly, the process is fully reversible, such that this previously undiscovered pathway can act like a switch, to help fine-tune a response to stress that potentially can lead to cell death.

"We hypothesize that the controlled production of H2S could have a profound impact on how this part of the ER stress pathway – the PERK 'arm' – is regulated. When proteins are misfolded in response to cellular stress, the inactivation and reactivation of PTP1B appears to be one means by which the cell regulates its protein synthesis machinery and can exert tight control over whether it lives or dies, ," says Tonks.

The linkage of such regulation with human disease is a subject that bears further exploration. ER stress is causally related to the protein-folding-related pathologies seen in such illnesses as Alzheimer's and Parkinson's diseases, Tonks says. "What we are trying to do is understand the structure of PTP1B in the presence and absence of its modification by H2S – to define this modification in molecular detail and understand its importance to the control of this major signaling enzyme in normal and disease states."

Hmm...go team go?

Brook Fri Dec 28, 2012 3:26 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°272

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 3:26 pm

Take a deep breath and read this:

H2S mediates O2 sensing in the carotid body

In adult mammals, carotid bodies are the sensory organs responsible
for monitoring arterial blood O2 concentrations and
relay sensory information to the brainstem neurons associated
with regulation of breathing and the cardiovascular system

Measurements of Respiratory Variables. Ventilation was monitored by whole-
body plethysmograph, and O2 consumption and CO2 production were de-
termined by the open-circuit method in unsedated mice as described in ref.
31. Ventilation was recorded while the mice breathed 21% or 12% O2-
balance N2. Each gas challenge was given for 5 min. O2 consumption and
CO2 production were measured at the end of each 5-min challenge. For
determining ventilatory response to CO2, baseline ventilation was de-
termined while mice breathed 21% O2 followed by hypercapnic challenge
with 5% CO2–21% O2–balance N2. Sighs, sniffs, and movement-induced
changes in breathing were monitored and excluded in the analysis. All
recordings were made at an ambient temperature of 25 ± 1 °C.

http://rfi.fmrp.usp.br/pg/fisio/cursao2012/H2S%20mediates.pdf

Read on at the link above...

Brook Fri Dec 28, 2012 3:33 pm

Brook

Brook: Posts : 3469
Join date : 2010-08-21
Age : 71

Post n°273

Re: Theater of parallel Universe

Brook Fri Dec 28, 2012 3:33 pm

High levels of 02 in the blood:

Oxygen toxicity

Oxygen toxicity is a condition resulting from the harmful effects of breathing molecular oxygen (O2) at elevated partial pressures. It is also known as oxygen toxicity syndrome, oxygen intoxication, and oxygen poisoning. Historically, the central nervous system condition was called the Paul Bert effect, and the pulmonary condition the Lorrain Smith effect, after the researchers who pioneered its discovery and description in the late 19th century. Severe cases can result in cell damage and death, with effects most often seen in the central nervous system, lungs and eyes. Oxygen toxicity is a concern for scuba divers, those on high concentrations of supplemental oxygen (particularly premature babies), and those undergoing hyperbaric oxygen therapy.

The result of breathing elevated concentrations of oxygen is hyperoxia, an excess of oxygen in body tissues. The body is affected in different ways depending on the type of exposure. Central nervous system toxicity is caused by short exposure to high concentrations of oxygen at greater than atmospheric pressure. Pulmonary and ocular toxicity result from longer exposure to elevated oxygen levels at normal pressure. Symptoms may include disorientation, breathing problems, and vision changes such as myopia. Prolonged or very high oxygen concentrations can cause oxidative damage to cell membranes, the collapse of the alveoli in the lungs, retinal detachment, and seizures. Oxygen toxicity is managed by reducing the exposure to elevated oxygen levels. Studies show that, in the long term, a robust recovery from most types of oxygen toxicity is possible.

Protocols for avoidance of hyperoxia exist in fields where oxygen is breathed at higher-than-normal partial pressures, including underwater diving using compressed breathing gases, hyperbaric medicine, neonatal care and human spaceflight.

http://en.wikipedia.org/wiki/Oxygen_toxicity

Hmmm...125 hits in a few hours....guess they did not get the memo....

should I say there's more?...